| 학회 |
한국공업화학회 |
| 학술대회 |
2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
26권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Proximity proteolysis reaction as a universal platform of biomarker sensing methods |
| 초록 |
Here, we report a one-step homogeneous assay method based on proximity proteolysis reactions (PPR) for detecting antibodies and small molecules. The proteolysis reaction between protease and zymogen was enhanced in the presence of the target, and the activated zymogen generated an absorbance signal by converting a chromogenic substrate. A protease and zymogen are linked to target binders using specific hybridization between complementary single-stranded DNAs. The developed assay methods successfully detected several kinds of analytes at subnanomolar concentrations. The one-step procedure and color signal of our PPR-based homogeneous assays will enable the development of simple point-of-care diagnostics for various types of biomarkers. The modular design of the PPR-based assay will enable the development of simple POC diagnostics for various biomarkers. |
| 저자 |
권민지, 유태현, 박현지, 이병준
|
| 소속 |
아주대 |
| 키워드 |
Biosensing; colorimetric; homogeneous assay; proximity; proteolysis
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| E-Mail |
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