| 초록 |
Addition of unnatural amino acids to protein used in various purposes. By orthogonal translation systems, unnatural amino acids are incorporated to target proteins. In modifying N-terminal position of protein, unnatual amino acid can be incorporated without affecting protein structure. But used orthognal translational pair, Methanococcus jannaschii(Mj) tyrosyl tRNA and aminoacyl-tRNA synthetase only recoginzes amber codons in elogation position. In this study, focusing on differences of initiator tRNA and elongator tRNA, we engineered Mj tRNA to bind formyltransferase and initiation factor 2 which participate in the assembly of aminoacylated initiator tRNA and new tRNA pair system incorporated unnatural amino acid at N-terminal position of target protein in overexpression of formyltrasferase. The engineered Mj tRNA / aminoacyl-tRNA synthetase pair could be used for protein engineering of incorporation of unnatural amino acid to N-terminal position. |
