| 학회 | 한국화학공학회 |
| 학술대회 | 2022년 봄 (04/20 ~ 04/23, 제주국제컨벤션센터) |
| 권호 | 28권 1호, p.915 |
| 발표분야 | [주제 10] 헬스케어 |
| 제목 | Application of lectin to detect virus by the modification of complex generation |
| 초록 | The properties of lectins allow them to bind to carbohydrates which is crucial in detecting receptor glycoprotein interactions. The structural and functional studies of concanavalin A (ConA), an investigated lectin, proved that it interacts with glucose and mannose. X-ray crystallography confirmed that ConA consists of two b-sheets and a short a-helix as a form of metalloprotein that is housed with Mn2+ and Ca2+ in the loop region. We have discovered the details of the interactions between ConA and HuNoV, that can facilitate the development of a sensitive detection method. Biophysical studies including HDX-MS & SPR demonstrated that the MCD is crucial for forming hydrogen bonds with carbohydrates. The specific hydrogen bonds were generated through CBD including Tyr, Asp, and Arg residues. The majority of interactions for the dimer are hydrogen bonds between two b-strands but the trimeric complex is regulated by the coordination with Cd2+. This study shows that ConA can generate diverse complexes including monomers, dimers, trimers, and tetramers based on the acidity, buffer conditions, and xenobiotic metal ions. |
| 저자 | 이채민1, 이승재2 |
| 소속 | 1Department of Chemistry, 2Jeonbuk National Univ. |
| 키워드 | 생물화공(Biochemical Engineering) |
| 원문파일 | 초록 보기 |
