| 학회 |
한국공업화학회 |
| 학술대회 |
2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
26권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Sensitive, One-step Fluorescence Onsite RNA Detection Based on Cell-free Synthetic Components |
| 초록 |
Recent outbreaks of infectious diseases arecaused by RNA viruses. Since RNA viruses are highly contagious and fatal, asensitive, simple, and rapid diagnostics assay is required to prevent the spread of viruses. In this study, we developed a one-step, cell-free assay for fluorescence-based RNA detection. It relies on an isothermal reaction cascade producing RNA aptamer which emits fluorescence as an output. The output aptamer is transcribed by T7 RNA polymerase from the ligation resultant of the two single-stranded DNA probes via SplintR ligase. We further sought to enhance ligation and transcription to improve the assay performance, including sensitivity and specificity. In addition, we also demonstrated that this assay could be used to detect emerging SARS-CoV-2 variants by leveraging the simplicity of the detection probe design. Overall, this isothermal one-pot, nucleic acid sequence-based diagnostics test can be a promising alternative to the standard molecular diagnostic method. |
| 저자 |
이정민, 우창하, 성도언, 장성호, 신기영, 이정욱, 정규열
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| 소속 |
POSTECH |
| 키워드 |
Synthetic Biology; Cell-free system; Molecular Diagnostics; Point-of-Care Test (POCT)
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| E-Mail |
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