| 학회 |
한국공업화학회 |
| 학술대회 |
2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 |
26권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Kinetic Separation in Escherichia coli by Developing Non-native Enzyme for Itaconate Production |
| 초록 |
Recently, microbial production of itaconatein Escherichia coli has been studied due to thecomplicated fermentation process and shortage of genetic engineering toolsof A. terreus. Unlike spatially compartmentalized itaconate synthesisof A. terreus, poor availability of its precursor, cis-aconitatehas been the major issue. In this study, directed evolution ofprotein known to have substrate promiscuity to citrate was conductedin E. coli to enhance the cis-aconitateavailability, with itaconate responsive screening system. Thereafter, themutant library was rationally designed and enriched. Finally,the developed mutant showed significantly enhanced catalytic efficiency tocitrate compared to the wild-type enzyme and consequently, its use enabledsignificantly increased itaconate production compared to the parental strain,indicating the successful kinetic separation between TCA cycle and itaconatebiosynthesis with the increase cis-aconitate supply for theitaconate production. |
| 저자 |
백선우, 예대열, 노명현, 문조현, 이정욱, 양재성, 정규열
|
| 소속 |
포항공과대 |
| 키워드 |
metabolic engineering; itaconate; kinetic separation; enzyme engineering
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| E-Mail |
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