| 초록 |
Advances in DNA nanotechnology have enabled the fabrication of DNA-based hydrogels with precisely controlled structures and tunable mechanical properties. Compared to DNA hydrogel, preparation of RNA hydrogel remains challenging due to its inherent instability. To overcome this limitations, we fabricated an aptamer-assisted RNA hydrogel via dual enzymatic polymerization. Multimeric short hairpin RNAs were hybridized with DNA aptamers for targeting and mechanical properties of the hydrogel. The obtained RNA hydrogel was ultrasoft and injectable hence reconfigurable into any confined structures. As a model system, RNA hydrogel was able to mimic microtubule structures, and designed to release the functional small interfering RNA (siRNA)-aptamer complex (SAC). In addition, we encoded restriction enzyme-responsive sites in RNA hydrogel to boost the release of SAC. This novel strategy provides an excellent platform for systematic RNA delivery, which has promising potential in RNA therapy. |
