| 학회 |
한국화학공학회 |
| 학술대회 |
2018년 가을 (10/24 ~ 10/26, 대구 EXCO) |
| 권호 |
24권 2호, p.2132 |
| 발표분야 |
[C1가스리파이너리사업단 국제 심포지엄] 바이오촉매관련(생물화공부문위원회) |
| 제목 |
Development of methanotroph strains converting methane to succinic acid with high efficiency |
| 초록 |
Recent development of mining technology enables to utilize CH4 in shale gas as a cheap and abundant feedstock. Succinate, which is a precursor for various chemicals such as tetrahydrofuran, γ-butylrolactone, 1,4-butanediol, is of our primary interest in this study. Mehtylomonas sp. DH-1 isolated from brewery plant sludge was suggested to have a strong potential for succinate production based on the existence of a full TCA cycle in the genome. In this study wild type and mutant strains were cultured in the fed batch reactor where mixture gas of methane and air was supplied continuously under either aerobic or O2-limited conditions. Under O2-limited condition the succinate production increased comparing to aerobic condition. When succinate dehydrogenase gene cluster (sdhB) was removed from the genome, ΔsdhB strain showed specific productivity ~ 60% higher than wild type despite of decreased cell growth. To overcome the growth defect of ΔsdhB, glyoxylate shunt genes were inserted into the genome. The mutant with glyoxylate shunt showed the highest succinate production, 176 mg/L. Further genetic manipulations are necessary to maximize succinate production of this strain. |
| 저자 |
김민식1, Susila Hadiyati1, Azka Nur Affifah1, 민경선1, Diep Thi Ngoc Nguyen2, 이상민1, 박권우1, 이수연1, 이준표1, 이진석1
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| 소속 |
1한국에너지기술(연), 2경희대 |
| 키워드 |
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| E-Mail |
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| 원문파일 |
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