| 초록 |
Targeting the oncogene that has a potential to induce cancer have been emerged as a therapeutic approach for the cancer treatment. CRISPR/Cas9 system is the most robust tools for the gene editing, modulating the target gene expression. However, an off-target effect the system reduces its efficacy on the organism and its effective delivery limits its utility for the clinical approach in vivo. Herein, we develop the highly efficient CRISPR/Cas9 delivery system for the cancer therapy in vivo based on biofunctional nanoparticles. The vehicles could efficiently load with the CRISPR/Cas9 complex, promoting the activation of the desired gene site and down-regulation of the oncogene protein level compared to conventional method. Therefore, this delivery system suggests a new approach on biomedical research and has a potential as a therapeutic application for genome-modified diseases in vivo. |
