| 학회 |
한국화학공학회 |
| 학술대회 |
2006년 가을 (10/27 ~ 10/28, 고려대학교) |
| 권호 |
12권 2호, p.1770 |
| 발표분야 |
생물화공 |
| 제목 |
Identification and characterization of glycosyltransferase gene from neomycin biosynthetic gene cluster |
| 초록 |
Neomycin produced by S. fradiae is among the clinically important 2-deoxystreptamine containing aminoglycoside antibiotics. Recently, the biosynthetic gene cluster for neomycin (Nem) was isolated and characterized from the neomycin producing strain, Streptomyces fradiae. The homology search with data base indicated that neo8 was glycosyltransferase in the neomycin (Nem) biosynthetic gene cluster. In the present study, we constructed disruption mutant of neo8, mutant that did not synthesize neomycin. But the complementation of mutant by neo8 experiments allowed us to determine that neo8 as glycosyltransferase gene from neomycin gene cluster. Here, it is first report that identification and characterization of glycosyltransferase gene form the aminoglycoside producer actinomycetes. |
| 저자 |
이희찬, Bimala Subba, Keshav Kumar Nepal, Hum Nath Jnawali, 류광경, 송재경
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| 소속 |
선문대 |
| 키워드 |
Aminoglycosides; Streptomyces fradiae; glycosyltransferase
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| E-Mail |
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| 원문파일 |
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