| 학회 |
한국공업화학회 |
| 학술대회 |
2007년 가을 (11/02 ~ 11/03, 한경대학교) |
| 권호 |
11권 2호 |
| 발표분야 |
생물공학 |
| 제목 |
Functional characterization of glycosyltransferase (kanF) gene in kanamycin gene cluster of Streptomyces kanamyceticus |
| 초록 |
The kanF (1164 nucleotides), located in the biosynthetic gene cluster of kanamycin producer, Streptomyces kanamyceticus, has putative function of glycosyltransferase. Multiple sequence alignment of amino acids shows 62 % and 33 % identities with already characterized neoD and btrM of Streptomyces fradiae and Bacillus circulans respectively. For the functional characterization of this gene, we attempted to over express in Escherichia coli BL21 (DE3) and the soluble fraction of protein was purified and used to carry out in vitro enzyme assay for glycosylation of UDP-N-Acetylglucosamine with 2-deoxystreptamine. |
| 저자 |
Nagendra Prasad Kurumbang, 이희찬, 류광경, 송재경
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| 소속 |
선문대 |
| 키워드 |
Glycosyltransferase; kanF; protein expression; Streptomyces kanamyceticus
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| E-Mail |
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