| 초록 |
The clustered regularly interspaced short palindromic repeat (CRISPR) system is one of the most powerful genome editing tools in biomedical research. The CRISPR system has been applied as a therapeutic, in most cases by viral vectors which show high in vivo transfection efficiency. However, viral delivery methods have been limited due to safety issues, therefore non-viral delivery strategies have been preferred. Herein, we introduce direct conjugates of the CRISPR-associated protein 9 (Cas9) with the amphiphilic and biocompatible lipid, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) to enhance the delivery efficiency with low toxicity. We prepared sgRNA targeting the epidermal growth factor receptor (EGFR) single mutation in cancer. After optimization, we hope that our approach would provide effective delivery of the Cas9 system for genome editing and open up new opportunities for clinical trials. |
