| 초록 |
The clustered regularly interspaced short palindromic repeat (CRISPR) system is one of the most powerful genome editing tools in biomedical research. The CRISPR system has been applied as a therapeutic, in most cases by viral vectors which show high transfection efficiency. However, viral delivery methods have been limited due to safety issues, therfore nonviral delivery strategies have been preferred. Herein, we conjugate the CRISPR-associated protein 9 (Cas9) with the amphiphilic and biocompatible lipid, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) directly, to enhance the delivery efficiency with same stability as native Cas9. We measured cytotoxicity of Cas9 complexes and genomed editing efficiency by using flow cytometry. After optimization, we hope that our approach would provide effective delivery of the Cas9 system for genome editing and open up new opportunities for clinical trials. |
