| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Development of Artificial Caprolactam Biosensor Using SELEX and in vivo Selection |
| 초록 |
Caprolactam is a monomer of highlighted material, nylon-6. RNA-encoded biosensor for caprolactam can facilitate high-throughput metabolic engineering of recombinant microbial strains. The unsolved issue in microbial production of caprolactam has been co-production of unwanted valerolactam in the recombinant strain. However, highly specific caprolactam biosensor has not been reported. Here, we developed an artificial riboswitch that specifically responds to caprolactam. This riboswitch was prepared using a coupled in vitro-in vivo selection strategy with a heterogeneous pool of RNA aptamers obtained from in vitro selection to construct a riboswitch library used in in vivo selection. The caprolactam riboswitch successfully discriminated caprolactam from valerolactam. It enabled caprolactam-dependent control of cell growth, which will be useful for improving caprolactam production and is a valuable tool for metabolic engineering. |
| 저자 |
김민선1, 장성연2, 장성호3, 임대균4, 강택진5, 오민규4, 정규열1
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| 소속 |
1포항공과대, 2Weil Cornell Medicine, 3인천대, 4고려대, 5동국대 |
| 키워드 |
Ribosiwtch; Caprolactam; SELEX
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| E-Mail |
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