| 학회 |
한국고분자학회 |
| 학술대회 |
2018년 봄 (04/04 ~ 04/06, 대전컨벤션센터) |
| 권호 |
43권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 I |
| 제목 |
Facile monitoring system of intracellular microRNA during adipogenesis via mechanical stimulus-induced exocytosis of liposomal probe |
| 초록 |
Non-invasive investigation of miRNA expression in culture soup is particularly useful for monitoring of stem cell differentiation without photo-toxicity of living cells, especially when cell morphologies remain unchanged during differentiation. However, real-time detection of miRNA in culture soup is not recommended because of insufficient miRNA amounts in culture soup. In this study, we introduce facile method for real-time assessing intracellular miRNA in culture soup by using liposomal miRNA beacon (Lipo-mB) and mechanical stimulus-mediated exocytosis. Pipetting-harvest of culture soup induces exocytosis-secretion of fluorescence signal of Lipo-mB from cytoplasm into culture soup. Lipo-mB successfully monitor stem cell differentiation (adipogenesis) by sensing changes in miRNA expression from culture soup of living cells. Lipo-mB can be further developed as an accurate sensing system for analyzing subtle differences in genotype, even when changes in phenotype cannot be observed. |
| 저자 |
한승민, 강병훈, 장은지, 기지선, 신무광, 정문영, 허용민, 손혜영, 함승주
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| 소속 |
연세대 |
| 키워드 |
liposome; miRNA sensing; real-time monitoring; differentiation; exocytosis
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| E-Mail |
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