| 학회 |
한국고분자학회 |
| 학술대회 |
2019년 가을 (10/09 ~ 10/11, 제주컨벤션센터) |
| 권호 |
44권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 II |
| 제목 |
Development and Evaluation of bioreducible gene carrier for peptide/gene co-delivery |
| 초록 |
(KLA)4 peptide has been well known as pro-apoptotic peptide, which could lead to anticancer effect through mitochondria penetration into cancer cell. However, it was limited in utilization because of the inefficient delivery in cancer cells. To solve these problem, bioreducible gene carrier (BGC), composed with (KLA)4 peptide, water-soluble chitosan (WC) and cystamine (Cys) was synthesized by coupling agent. Its chemical structure was analyzed by 1H-NMR. The complex was prepared by electrostatic charge interaction. The particle size, zeta potential and morphological property of BGC/gene complexes were comfirmed by DLS and TEM. In addition, its binding affinity, DNase protection and releasing assay were carried out by gel retardation assay. Moreover, its transfection efficacy was evaluated in diverse cancer cell lines. These results suggest that it is superb gene carrier with targeted mitochondria and nucleus into cancer cells. |
| 저자 |
김준수1, 홍웅길1, 안준혁1, 정경원2, 박준규3, 심왕근1, 나재운1
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| 소속 |
1순천대, 2조선이공대 생명환경화공과, 3(주)시지바이오 |
| 키워드 |
(KLA)4 peptide; Gene; Water-soluble chitosan; Bioreducible; Anticancer effect
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| E-Mail |
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