| 학회 |
한국공업화학회 |
| 학술대회 |
2020년 가을 (10/28 ~ 10/30, 광주 김대중컨벤션센터(Kimdaejung Convention Center)) |
| 권호 |
24권 1호 |
| 발표분야 |
포스터-생체재료 |
| 제목 |
Development of gene and peptide carriers for the treatment of cancer cell. |
| 초록 |
(KLA)4 peptide penetrates the mitochondria of cancer cell. It had an anti-cancer effect. However, its one-self had a low delivery efficiency and low anti-cancer effect of cancer cells. To solve these problem, the non-viral vector chitosan and (KLA)4 peptide and Cystamine were synthesized using coupling agents. Its chemical structure was analyzed by 1H-NMR. peptide/gene complex was prepared by electrostatic charge interaction. Its binding affinity, DNase protection and releasing assay were carried out by gel retardation assay. Its transfection efficacy was evaluated in diverse cancer cell lines. Besides, its cytotoxicity and anticancer effect were conducted by MTT assay. These results suggest that it is superb gene carrier with targeted mitochondria and nucleus into cancer cells. |
| 저자 |
김준수1, 홍웅길1, 안준혁1, 정경원2, 심왕근1
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| 소속 |
1순천대, 2조선이공대 |
| 키워드 |
(KLA)4 peptide; Gene; chitosan; Anticancer effect
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| E-Mail |
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