| 학회 |
한국화학공학회 |
| 학술대회 |
2022년 봄 (04/20 ~ 04/23, 제주국제컨벤션센터) |
| 권호 |
28권 1호, p.755 |
| 발표분야 |
[주제 5] 친환경 플라스틱 |
| 제목 |
A Plug-in Repressor Library for Precise Regulation of Metabolic Flux in Escherichia coli |
| 초록 |
In metabolic engineering, improved production of value-added chemicals requires metabolic flux optimization. Although advances in synthetic biology have achieved the exploitation of a number of genetic elements for precise flux control, their use requires expensive inducers, needs complex and time-consuming processes to design and optimize conditions. To overcome this issue, we devised a plug-in phlF repressor library for target-specific flux control, where its expression level was diversified using degenerate 5’ untranslated region (5’ UTR) sequences generated by the UTR Library Designer. A validated wide range of repressor library was applied to enhance the production of 3-hydroxypropionic acid (3-HP) and lycopene in Escherichia coli. Consequently, we successfully achieved carbon flux optimization for efficient production. The most optimized strains produced 1.08 g/L of 3-HP and 10.6 mg/L of lycopene, which were improved 5.5-fold and 1.9-fold, respectively, compared to the parental strains. These results indicate that the repressor library is a powerful tool for efficient production of value-added chemicals by fine-control of carbon flux. |
| 저자 |
민명원, 이지연, 차상학, 이지훈, 임현규, 노명현, 강채원, 정규열
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| 소속 |
포항공과대 |
| 키워드 |
생물화공(Biochemical Engineering) |
| E-Mail |
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| 원문파일 |
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