| 학회 |
한국화학공학회 |
| 학술대회 |
2022년 봄 (04/20 ~ 04/23, 제주국제컨벤션센터) |
| 권호 |
28권 1호, p.1224 |
| 발표분야 |
[주제 12] 화학공학일반(부문위원회 발표) |
| 제목 |
Kinetic Flux Separation in Escherichia coli by Non-native Enzymatic Reaction for Itaconate Production |
| 초록 |
Microbial production of itaconate has been studied in Escherichia coli due to the complicated fermentation process and shortage of genetic engineering tools of Aspergillus terreus. One of the major issues on itaconate production in industrial strains is the poor availability of its precursor, cis-aconitate, unlike spatially compartmentalized itaconate synthesis of A. terreus. In this study, directed evolution of protein known to have substrate promiscuity to citrate was conducted in E. coli to enhance the cis-aconitate availability. Initially, the itaconate responsive screening system could be constructed by using the itaconate-responsive transcription factor (ItcR) from Yersinia pseudotuberculosis. Thereafter, the mutant library was rationally designed and enriched. Finally, the developed mutant showed significantly enhanced catalytic efficiency to citrate compared to the wild-type enzyme and consequently, its use enabled significantly increased itaconate production compared to the parental strain, indicating the successful kinetic separation between TCA cycle and itaconate biosynthesis with the increase cis-aconitate supply for the itaconate production. |
| 저자 |
예대열1, 노명현1, 문조현1, 이정욱1, 양재성2, 정규열1
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| 소속 |
1포항공과대, 2CRAG |
| 키워드 |
생물화공(Biochemical Engineering) |
| E-Mail |
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| 원문파일 |
초록 보기 |
