| 초록 |
Effective delivery of interleukin (IL)-15 to natural killer (NK) cells and activation of their anticancer efficacy could be a promising cancer immunotherapy. For the protection of IL-15 in body environments, coacervate (Coa) (complex of (1) methoxy polyethylene glycol-poly(ethylene arginyl aspartate diglyceride), a cationic polymer, (2) heparin, anionic polymer, and (3) cargo IL-15) were developed. mPEGylation successfully protected colloidal structures of Coa from harsh environments and controlled release profile of IL-15. After culture with IL-15 loaded Coa, proliferation of NK cells was enhanced as compared with bolus IL-15 treatment. mRNA expression profiles related to anticancer efficacy of primed NK cells were also upregulated. In addition, co-culture with NK cells and multiple cancer cell lines, Coa treated group exhibited facilitated anticancer efficacy. In summary, Coa-mediated exogenous IL-15 delivery could be an effective NK cell priming technique for cancer immunotherapy. |
