| 초록 |
In metabolic engineering, improved production of value-added chemicals requires precise flux regulation between growth-essential competing and production pathways. Although advances in synthetic biology have achieved the exploitation of a number of genetic elements for precise flux control, their use needs complex and time-consuming processes to design and optimize its expression level for each individual. To overcome these, we devised a plug-in repressor library for target-specific flux control. After we validated a wide expression range of the repressor library, it was applied to enhance the production of 3-HP from acetate, lycopene from glucose in E. coli via precise flux rebalancing. The most optimized strains produced 1.08 g/L of 3-HP and 10.6 mg/L of lycopene, which were improved 5.5-fold and 1.9-fold, respectively, compared to those produced by the parental strains. Consequently, we successfully achieved optimal carbon fluxes around the precursor nodes for efficient production. |
