| 학회 |
한국화학공학회 |
| 학술대회 |
2021년 가을 (10/27 ~ 10/29, 광주 김대중컨벤션센터) |
| 권호 |
27권 2호, p.1723 |
| 발표분야 |
생물화공 |
| 제목 |
CRISPR-Cas12a-assisted cell-free DNA biosensor based on Metal-Enhanced Fluorescence and Colorimetric Analysis |
| 초록 |
Cell-free DNA (cfDNA) can be used as a biomarker for cell death and defects due to its low concentration in circulating blood. However, application of cfDNA in clinical fields is not easy because of difficulty in nucleic acid amplification step. In this study, nucleic acid amplification free-CRISPR-Cas12a-assisted cfDNA biosensor was fabricated using DNA-associated gold nanoparticle (GNP) for a metal-enhanced fluorescence (MEF). The fabricated cfDNA biosensor was designed to cleave single-stranded DNA (ssDNA) between FITC and larger GNP when presence of a target cfDNA (BRCA-1). The dissociation of the larger GNP from FITC changed color from purple to red-purple. As a result, this cfDNA biosensor can detect BRCA-1 presenting high sensitivity (as low as 0.34 fM) and rapid (<30 min) detection. The developed method can be adapted to quantify levels of other nucleic acids as a promising diagnostic and prognostic strategy in a field-deployable platform. Acknowledgments: This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2019R1A2C3002300) and by the Ministry of Education (No. 2016R1A6A1A03012845). |
| 저자 |
안주현1, 최진하2, 임종표1, 신민규1, 최정우1
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| 소속 |
1서강대, 2전북대 |
| 키워드 |
생물화학공학 |
| E-Mail |
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| 원문파일 |
초록 보기 |
