| 초록 |
Branched polyethyleneimine (PEI) was well known as high gene transfection agent, However, utilization of PEI as gene carrier was limited because of high cytotoxicity. To solve this problem, PEI was introduced to chitosan (CS) back bone by coupling agent. Also, hyaluronic acid(HA) was introduced for targeted CD44-receptor in breast cancer cell. Structural analysis of PEI-grafted chitosan (CSPEI) was confirmed by 1H-NMR. In addition, ternary complex of pDNA/CSPEI/HA was prepared by electrostatic charge interaction. Its transfection assay and cellular uptake showed that ternary complexes were enhanced more than binary complexes by targeting effect of HA. These results suggest that it can enhanced to efficacy of gene delivery by targeted CD44-receptor in breast cancer cell. This work was supported by National Research Foundation of Korea (NRF) grant funded by the Ministry of Science, ICT & Future Planning. (No. NRF-2014R1A2A1A10053027). |
