| 초록 |
This study was aimed at profiling of proteins in exosomes to identify lung cancer biomarkers. Exosomes were isolated from 100 serum samples via PEG-based precipitation and immunoaffinity separation using CD9, CD63, CD81, and EpCAM antibodies. The proteomic analysis was performed using 2-D gel electrophoresis and MALD-TOF/mass spectroscopy sequentially. Differentially expressed proteins (DEPs) increased in lung cancer samples were selected and their expression was validated by western blotting for exosomes and cancer tissues. A total of 55 protein spots were selected for increased DEPs, and CLEC3B, ITIH4, SERFINF1, SAA4, SERFINC1, and C20ORF3 were increased in patient-derived exosomes. Proteomic profiling of exosome proteins could reveal potential biomarkers for lung cancer diagnosis. They showed high AUC and coincided expression in exosomes and cancer tissues. The exosome-derived proteins are proposed as biomarkers of liquid biopsy for noninvasive diagnosis of lung cancer. |
