| 학회 |
한국고분자학회 |
| 학술대회 |
2014년 봄 (04/10 ~ 04/11, 대전 컨벤션센터) |
| 권호 |
39권 1호 |
| 발표분야 |
휴먼인터페이스 의료용 고분자재료 (의료용 고분자 부문위원회) |
| 제목 |
Heterogeneous transfection method based on encoded microparticle. |
| 초록 |
We designed an encoded micropatch immobilizing viral vector. The encoded micropatches are placed on the cell layer and infect the cells with viral vector. We used graphical code for encoding scheme, which is simple but powerful. Key feature of encoded micropatch is local gene delivery with code according to the shape of micropatch. Various shapes of micropatch were used for viral transduction. We used ad-RFP viral vector, which can deliver a gene expressing RFP in a cell. We found RFP expressed cell with micropatch’s shape. By using this feature, heterogeneous genes were locally delivered to cells. We fabricated heterogeneous micropatches immobilized with GFP and RFP expressing gene and randomly spread them on cells cultured well plate. Heterogeneous gene was successfully delivered by using viral vector micropatches and they express GFP and RFP in cell area covered by each encoded micropatch. |
| 저자 |
박욱1, 권성훈2, 한상권2, 배형종2
|
| 소속 |
1경희대, 2서울대 |
| 키워드 |
microparticle; gene delivery
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| E-Mail |
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