| 초록 |
Naringenin is one of the high value-added compounds in that it is a key precursor for diverse flavonoids and has pharmaceutical activities by itself. Here, bioinformatics-based fine-tuning tools to control multiple genes expression at both transcription and translational levels were thoroughly studied. As a further step, pathway optimization was finally conducted through screening the mutant libraries based on synthetic RNA devices with a high-throughput screening capability. Through this systematic strategy, a mutant with naringenin productivity 2.97 times higher than the control strain. It showed 260.2 mg/L naringenin titer, which is the highest value reported so far among glycerol-based production in Escherichia coli. We demonstrate that this integrative optimization at transcriptional and translational levels based on the biosensors is one of efficient and powerful strategy for optimization of multi-gene expression levels. |
