| 학회 |
한국화학공학회 |
| 학술대회 |
2013년 가을 (10/23 ~ 10/25, 대구 EXCO) |
| 권호 |
19권 2호, p.1530 |
| 발표분야 |
생물화공 |
| 제목 |
Biosynthesis of 2-hydroxybutyrate containing polyhydroxyalkanoates by recombinant Escherichia coli |
| 초록 |
As global concerns on environmental problems and availability of petroleum resources increase, production of biomass-driven chemicals has attracted much more attentions. Polyhydroxyalkanoates (PHAs) is one of the most promising alternatives of chemically synthesized polymers having mechanical properties that are similar to those of petroleum-based plastics and they can be easily modified by altering monomer compositions. Biological synthesis of PHA is composed of two distinguished steps. Firstly, hydroxyacyl-CoAs which are the monomers of PHA are produced by natural and engineered microbial strains. Secondly, the PHA synthase, which is the key enzyme in PHA synthesis, accepts its specific monomers and then synthesize PHAs. Here, we report recombinant E. coli strains expressing Clostridium propionicum propionyl-CoA transferase (PctCp) and Pseudomonas sp. 6–19 PHA synthase (PhaC1Ps6–19) genes that are able to produce 2HB-containing PHAs using propionyl-CoA as precursor for 2HB monomers. Detailed results will be presented in this presentation. |
| 저자 |
오영훈1, 박시재2, 강경희2, 양정은3, 송봉근1, 제갈종건1, 이승환1, 이상엽3
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| 소속 |
1한국화학(연), 2명지대, 3한국과학기술원 |
| 키워드 |
2-hydroxybutyrate; PHA; biosynthesis
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| E-Mail |
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| 원문파일 |
초록 보기 |
