| 초록 |
In this study, we prepared the silica based inverse opal structure for detection of influenza virus. It has 3-dimentional nanostructure, which is composed of spherical void spaces with solid walls periodically. This structure has the optical response due to its periodic arrangement. It can be applied to catalysis, battery, solar cell, and bioactive materials through the high accessible surfaces and porous structure. Because of these benefits of the 3D-nanostructure, it is suitable for bio-sensing. We attempt to fabricate the inverse opal-based biosensor and observe the optical response for detecting biological targets. We prepared opal structure by evaporation of colloidal solution for well-ordered structure. And then, this opal structure was calcinated at 500℃ for removing colloids. Then inverse opal nanostructure was treated by 3-aminopropyl trimethoxysilane (APTMS) to conjugate biomolecules and maleimide linker and protienG for antibody immobilization. Optical response of inverse opal structure appears when biological targets detected. The sensitivity and specificity are analyzed by reflectance variation. We can detect only 10ul of influenza virus from 103 PFU to 105 PFU without label. This study will provide various sensing strategies through surface functionalization of inverse opal nanostructure. |
