| 초록 |
Acetate is a final product of natural anoxic biodegradation of many organic compounds and waste. The accumulation of acetate hinders further fermentation and biodegradation in many bioprocesses. However, the utilization of acetate in bioprocess has not been extensively studied. In this study, we tried to develop a strain and process to convert acetate into more value-added platform chemicals such as succinate which is intermediate for further synthesis of bio-based plastics, solvents, adhesives, and resins. We attempted to convert acetate using an industrially applicable strain, Pseudomonas putida under microaerobic conditions via aerobic pathways. To improve acetate assimilation as well as succinate production, the branch point enzyme isocitrate lyase which converts isocitrate to glyoxylate and succinate was homologously overexpressed in P. putida. Further, succinate dehydrogenase deletion was conducted to inhibit the succinate reassimilation by bacteria. This study provides a platform to understand the role of isocitrate lyase to produce bio-succinic acid from refractory C2 carbon such as acetate under microaerobic conditions. |
