| 초록 |
On-site genetic detection needs to develop a sensitive and straightforward biosensor without special equipment which can detect various genetic biomarkers. Here, we developed a novel genetic biosensor based on enzyme-free dual-amplification of universal hybridization chain reaction (uHCR) and hemin/G-quadruplex horseradish peroxidase (HRP)-mimicking DNAzyme. The uHCR is the strategy that enables simple design for multiple target detection by introducing a target-specific trigger hairpin without changing the whole system according to a target change. Also, HRP-mimicking DNAzyme could produce a sensitive and quantitative colorimetric signal with stability. As a result, the uHCR biosensor could universally detect four different targets for cancer diagnosis. In addition, the uHCR biosensor could discriminate single nucleotide polymorphism even in the serum. Overall, the uHCR biosensor demonstrated the potential for field testing with a simple redesign without complicated steps or special equipment using a universal hairpin system and enzyme-free amplification. Therefore, it could be applied to urgent detection of various pathogens, remote diagnosis, and self-screening of diseases. |
