| 학회 |
한국고분자학회 |
| 학술대회 |
2021년 가을 (10/20 ~ 10/22, 경주컨벤션센터) |
| 권호 |
46권 2호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
Highly sensitive exosomal miRNA detection system based on successive toehold-mediated DNA strand displacement within fusogenic vesicles |
| 초록 |
MicroRNAs(miRNAs) are promising diagnostic biomarkers, involved in gene regulation process associated with various diseases. Particularly, miRNA from tumor-derived exosomes play pivotal role in noninvasive cancer diagnosis, since tumor exosomes are highly enriched in body fluids. However, current methods of exosomal miRNA detection require substantial amount of time and expense. Here, we propose detection system consisting of two major procedures; membrane fusion and non-enzymatic target recycling signal amplification. Membrane fusion is induced by specific binding between Influenza virus-derived Hemagglutinin(HA) proteins on fusogenic vesicle and sialic acid on exosome. Upon successful vesicle fusion, miR-10b-activated DNA molecular machine generates amplified fluorescence signal by recycling target miRNA, contributing to the enhanced sensitivity. Therefore, the proposed method is expected to be remarkably time-saving and cost-cutting miRNA detection technique. |
| 저자 |
정수현, 박채원, 박근선, 이소정, 임종우, 함승주
|
| 소속 |
연세대 |
| 키워드 |
exosomal miRNAs; membrane fusion; toehold-mediated DNA strand displacement; miRNA detection; exosome detection
|
| E-Mail |
|
