| 초록 |
We herein developed a rapid and novel isothermal amplification method to detect platelet-derived growth factor (PDGF) utilizing nicking and extension chain reaction system-based amplification (NESBA). In principle, the aptamer strand (AS) specifically binds to PDGF-BB. The binding of PDGF-BB to AS induces the release of template strand (TS) which was designed to contain 5’ phosphorothioate (PS)-modified target recognition site. Then, TS initiates the following NESBA system, leading to the exponential amplification of RNA amplicons that are monitored by molecular beacon (MB). With this strategy, we sensitively determined a model target, PDGF-BB, down to 6.48 nM with high selectivity against non-specific analytes including different types of PDGFs. More importantly, we also demonstrated the capability of this sensor to reliably analyze PDGF-BB in the human serum sample, verifying its practical applicability to the development of point-of-care (POC) device for cancer diagnosis. |
