| 학회 |
한국화학공학회 |
| 학술대회 |
2007년 봄 (04/19 ~ 04/20, 울산 롯데호텔) |
| 권호 |
13권 1호, p.1262 |
| 발표분야 |
분자인식기반 고도분리기술 심포지엄 |
| 제목 |
Development of Carousel Ion-exchange Processes for the Removal of Zinc Ions from a Protein Mixture |
| 초록 |
Chelex 100 is a highly effective adsorbent for the separation of metal ions from proteins for three reasons; it has a high affinity for metal ions; proteins with molecular weight of 1,000 or higher are excluded from the particle pores; it also allows high flow rates because it can stand a pressure drop up to 100 psi. In this study, a carousel process based on Chelex 100 has been developed for the removal of zinc ions from a protein solution. The intrinsic parameters for the carousel design were estimated from a series of single column experiments, which showed that Chelex 100 has a high selectivity for zinc ions in 1 N acetic acid and it can be effectively regenerated using 0.1 N HCl. The exchange mechanisms between Zn2+ and H+ on Chelex 100 were studied and considered in rate model simulations. The effective zinc isotherm was found to be unfavorable in 1 N acetic acid. A design method based on the Standing Wave Analysis for unfavorable isotherm systems has been developed in this study to ensure high product purity and high yield in carousel ion-exchange processes. |
| 저자 |
문성용 |
| 소속 |
한양대 |
| 키워드 |
Ion-exchange; Carousel; Separation
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| E-Mail |
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| VOD |
VOD 보기 |
| 원문파일 |
초록 보기 |
