| 초록 |
It is important to monitor the concentration of hydrogen sulfide (H2S), an endogenous gas transmitter which plays a crucial role in a wide range of biological and chemical processes in living organisms, for the detection of certain diseases such as alzheimer’s disease, down syndrome and diabetes. Ratiometric fluorescent probes have brought significant attention due to easy monitoring the biological active molecules. Herein, we designed a ratiometric fluorescent probe (sNBD-Pz-R5) consisting of two fluorophores, sNBD and coumarin for the FRET system. Furthermore, we investigated the role of arginine amino acids to the probe for cell penetration and reactivity for H2S. Initially in this system the FRET is on and after the addition of hydrogen sulfide, sulfide ion attacks the nitrogen of piperazine and sNBD group is detached from the sensor. Then FRET system is off from which ratiometric response can be observed. |
