| 초록 |
Controlling molecular transport is significantly important for purification, separation, and compartmentalization, all of which are highly useful techniques for many applications. In particular, if size-selective membranes are available to regulate the transport of various molecules in a clinical sample, they hold great potential for point-of-care tests in detecting desired analytes in body fluids; by simply excluding fouling molecules, the membrane-protected probes maintain their best performance in analyte detection even in a non-treated complex mixture. In this work, we employed a water-in-oil emulsion process to construct the protein-polymer microcapsules by PNIPAm-albumin self-assembly and their cross-linking. We then encapsulated aptasensors that can fluorescently report the presence of various biomarkers, such as estradiol, dopamine, and cocaine, demonstrated that the aptasensor-encapsulated protocells can serve as high-performance biosensors, even in undiluted blood and serum. |
