| 학회 | 한국공업화학회 |
| 학술대회 | 2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 | 26권 1호 |
| 발표분야 | 포스터-생체재료 |
| 제목 | Efficient Gene Delivery system using Hyaluronic Acid/Calcium PhosphateHybrid for osteogenic differentiation of Human Mesenchymal Stem Cells |
| 초록 | Human mesenchymal stem cells (hMSC), one ofthe stem cells, have multipotent properties. Despite their boundless possibility,current use ofhMSCs in clinics is still limited because differentiation of hMSCs to desiredcells is often difficult to control especially in vivo situation. Herein, biologicallyacceptable calcium phosphate (CaP)-based nanoparticles stabilized with acatechol-derivatized hyaluronic acid (dopa-HA) conjugate were used as a carrierfor gene transfection to hMSCs for improved differentiation. Owing to the specificbinding between HA and CD44 of bone marrow-derived hMSCs, dopa-HA/CaP showedsignificantly higher transfection in hMSCs than branched polyethylenimine(bPEI, MW 25 kDa) with high biocompatibility. The co-delivery of a plasmid DNAencoding bone morphogenetic protein 2 (BMP-2 pDNA) and micro RNA 148b (miRNA-148b)by dopa-HA/CaP successfully achieve improved osteogenic differentiation ofhMSCs. |
| 저자 | 박채은, 임수연, 김다훤, LYU SIYAN, 황위보, 김병덕, 정준구, 신주화, 이지현, 유빈, 정지훈 |
| 소속 | 성균관대 |
| 키워드 | Gene Delivery system; nucleic acid; Delivery system |
