| 학회 | 한국화학공학회 |
| 학술대회 | 2022년 봄 (04/20 ~ 04/23, 제주국제컨벤션센터) |
| 권호 | 28권 1호, p.1237 |
| 발표분야 | [주제 12] 화학공학일반(부문위원회 발표) |
| 제목 | High-level production of heme using metabolically engineered Escherichia coli by fed-batch fermentation |
| 초록 | An important prosthetic group, heme, has broad applications in health functional food industries. We reported metabolically engineered Escherichia coli strain that produces heme from glucose and L-glutamate. In this study, we optimized the fermentation conditions to achieve enhanced production of heme. Firstly, the component and concentration of culture medium was screened at flask level, and then optimized the other factor and culture conditions during fed-batch fermentation process. In conclusion, the titer and productivity of heme production during fed-batch fermentation were improved several times. The optimization strategies employed here is applicable for the high-level production of bio-based production of value-added chemicals and high-quality alternative meats. This work was supported by the “Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ01577901)” from Rural Development Administration, Republic of Korea. |
| 저자 | 유혜은, 최경록, 이상엽 |
| 소속 | 한국과학기술원 |
| 키워드 | 생물화공(Biochemical Engineering) |
| 원문파일 | 초록 보기 |
