| 학회 |
한국고분자학회 |
| 학술대회 |
2022년 봄 (04/06 ~ 04/08, 대전컨벤션센터) |
| 권호 |
47권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Surface charge-mediated nucleic acid extraction and purification using a positively-charged copolymer synthesized via iCVD |
| 초록 |
The rapid and sensitive diagnostics of viral infection throughout nucleic acid-based methods, such as PCR, has been significant as the viruses are spreading on a global scale. However, the detection methods rely on extracting the nucleic acid from the patients, which requires multiple liquid handling and centrifugation steps. Herein, we develop a single-step RNA extraction method using charge interaction between the nucleic acid and positively charged copolymer synthesized via iCVD. A tertiary aminated DMAMS was copolymerized with varying degrees of epoxy-containing GMA to design the copolymer. Successful copolymerization was confirmed by FTIR, XPS, and contact angle measurement. The series of copolymer films were deposited on the surface of the PCR tube and applied to bind and isolate nucleic acid through the charge interaction covalently. The contaminants, including proteins and cellular components, were reacted with GMA and assisted nucleic acid purification from the specimen. |
| 저자 |
최윤영1, 송윤성1, 김민아2, 임성갑1, 성혜정3
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| 소속 |
1KAIST, 2KU-KIST, 3KIST |
| 키워드 |
nucleic acid; extraction; detection
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| E-Mail |
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