| 초록 |
Hybrid hydrogel of natural and synthetic hydrogel is required to complement the disadvantages of each and to mix two hydrogels into one, one-pot double cross-linking is preferred to multi-step double cross-linking. Adipose derived stem cell (ADSC) is known to differentiate into adipocyte when the mechanical strength of the matrix decreases during cell culture. Here, we present one-pot double cross-linked PEG-Collagen hydrogel that has rheology-decreasing property for ADSC culture/delivery system. Disulfide bond in PEG hydrogel is cleaved by ADSC-released glutathione (GSH), contributing to rheology-decreasing property. It has been observed that PEG and Collagen hydrogel do complement each other. PCR measurement showed that PEG-Collagen hydrogel enhances ADSCs differentiation into adipocytes. By applying GSH and Collagenase, adipocytes are retrieved from the hydrogel. This hydrogel is newly introduced as ADSC differentiation platform and can be applied to other cell type therapy. |
