| 초록 |
Vitamin C, has emerged as an alternative to conventional chemotherapy for treating cancers because of its oxidative stress promotion. However, vitamin C should be delivered to cells in its oxidized form, dehydroascorbate (DHA) due to its cytotoxicity. By incorporating diselenide-based amphiphilic lipids into the liposomal membrane, Glutathione (GSH)-responsive nanoparticles (G-NPs) have been prepared as potential carriers of DHA. DHA was wrapped into NPs using the dehydrationrehydration method to make DHA-loaded G-NPs which induced much higher GSH depletion in HT29 colon cancer cells than DHA-loaded controls. Particularly, DHA-contained G-NPs sharply increased the intracellular levels of reactive oxygen species, implying enhanced oxidative stress by stimuli-responsive DHA release. As a result, DHA-contained G-NPs exhibited significant cytotoxicity against HT29 cells. Overall, G-NPs might serve as emerged deliver for intracellular delivery of DHA during cancer treatment. |
