| 초록 |
Various types of stem cells such as human adipose derived stem cells (hADSCs) for biomedical application can undergo cellular damage due to an enzymatic process to collect cells. Herein, we have proposed an alternative method for delivering hADSCs with pH-sensitive polymer layer without enzyme treatment. The hADSCs showed no statistical differences in cell adhesion, proliferation, viability, and apoptotic activity compared to conventional enzyme treated cells. However, the hADSCs released more angiogenic factors under serum free hypoxic condition. Indeed, the hADSCs showed improvement in therapeutic angiogenesis when delivered to in vivo mouse hindlimb ischemia model compared to traditional cell injection method using enzyme for cell collection. |
