| 초록 |
In some cases, virus variants accompany noticeable changes to its phenotype. The mutants can paralyze the disease surveillance and response system, as we have been experienced with the Omicron variant. In order to efficiently cope with virus variants, a fast discrimination assay is required that it is not only simply designable, but easily available on-site. In this study, we developed a virus variant discrimination method, based on a standard PCR amplicon. The assay relies on ligation-dependent SNP discrimination, where the ligation only proceeds when each probe is completely complementary to a target sequence. This assay was able to discriminate the Omicron variant from the SARS-CoV-2 and vice versa in 20 min. Our diagnostics assay can distinguish an SNP-level mutation rapidly, without the need for specialized instruments, showing potential as a field-deployable technology. Due to the simplicity of probe design, the assay can be rapidly built when a new variant outbreaks. |
