| 초록 |
Majority of SNP detection kits rely on expensive instruments such as real–time PCR or NGS system. Therefore, it has been still challenge to develop a cost effective and less instrument dependent genetic test. Ligase Chain Reaction (LCR) is one of the most specific methods for the point mutation. However, LCR is also thermal cycler dependent. Therefore, in this presentation, isothermal multiple ligase reaction per single target DNA will be presented. For the multiple ligase reaction, strand displacing oligonucleotide (SDO) was introduced into the reaction. After two primers were ligated upon hybridization with target, the template could be displaced with SDO and recycled. The isothermal ligase reaction was combined to the modified cycling probe assay, an method to amplify fluorescence signal. As a result, point mutations in KRAS as low as attomole gene could be detected. |
