| 초록 |
Recently, there are increasing social and ethical concerns regarding the use of animal experiment for property test of new chemicals. In this study,we developed biomimetic dual-functional scaffolds(EG-AP)using radiation technology, and investigate the regulation of hMSC fate on them. Poly(L-lactide-co-ε-caprolactone) (PLCL) scaffolds were fabricated by electrospinningmethod, and then acrylic acids(AAc) were grafted by gamma-ray irradiation. To increase biological function of the scaffolds, EGF and gelatin were co-immobilized on the scaffolds, and the characteristics of the scaffolds were analyzed by SEM, TBO uptake, and ATR-FTIR. During the hMSC culture, adhesion and proliferation of hMSCs were significantly increased on the EG-AP . Furthermore, we confirmed that differentiation of hMSC was up-regulated on the EG-AP scaffolds under normal culture condition. Therefore, our biomimetic strategy using gamma-ray irradiation may present using alternativetools in skin tissue engineering. |
