초록 |
Hydrogen is regarded as potential alternative energy sources because it is renewable, has high energyyield. Biohydrogen has been gaining attention for itspotential as a sustainable alternative to the existing hydrogen productionmethods because it had a simpler, cleaner, and cheaper producing methods. For efficient biohydrogen production, Clostridium sp. SH25 was isolated from anaerobic sludge obtained from a wastewater treatmentplant. Glycerol was identified suitable carbon source. Also, optimal initial glycerol concentration, temperature, initial pH, NaCl concentration, and inoculum size were determined. Under these optimized conditions, Clostridium sp. SH25 could produce biohydrogen from crude glycerol. In addition, the potential application ofbiohydrogen derived from crude glycerol was demonstrated in a educational model hydrogencar. In conclusion, Clostridium sp. SH25 successfully produced biohydrogenusing crude glycerol for application as fuel in hydrogen cars. |