| 학회 | 한국고분자학회 |
| 학술대회 | 2004년 가을 (10/08 ~ 10/09, 경북대학교) |
| 권호 | 29권 2호, p.683 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Design of Extracellular Matrix for Liver Tissue Engineering |
| 초록 | The cellular environment is dominated by the extracellular matrix (ECM), which is mainly composed of collagens, proteoglycans, and glycosaminoglycans (GAGs) that are secreted and assembled locally into an organized network to which cells attach. Interest in the ECM has increased dramatically in recent years due to an increasing awareness of its relevance to the regulation of cell growth, morphology, and differentiation. Heparin as one of species of the GAGs, is anionic polysaccharide and can bind tightly the GAGs integration site and become a “bridge” between cells and materials [1]. Chitosan as a binary heteropolysaccharide containing [1-4] linked 2-acetamide-2-deoxy-ß-D-glucopyranose and 2-amino-2-deoxy-ß-D-glucopyranose residues has characteristics similar to GAGs and is biodegradable and non-toxic. In this study, galactose moiety was covalently coupled with chitosan for hepatocytes attachment and then, heparin was immobilized into the galactosylated chitosan (GC) to enhance hepatocytes functions. The synthetic scheme of GC-heparin (GCH) was shown in Fig. 1. Hepatocytes attachment and DNA synthesis of hepatocytes attached onto GCH will be reported. [1]. J.L. Li, J.L. Pan, L.G. Zhang, X.J. Guo, Y.T. Xu, J. Biomed Mater Res. 67, 938 (2003). [2]. I.K. Park, J. Yang, H.J. Jeong, H.S. Bom, I. Harada, T. Akaike, S.I. Kim, C.S. Cho, Galactosylated chitosan as a synthetic extracellular matrix for hepatocytes attachment, Biomaterials 24, 2331 (2003). |
| 저자 | 강호림1, 서석진1, T. Akaike2, T. Hoshiba3, 조종수2 |
| 소속 | 1서울대, 2Tokyo Institute of Technology, 3Japan |
| 키워드 | ECM; liver; chitosan; galactose; heparin |
