| 학회 |
한국고분자학회 |
| 학술대회 |
2021년 봄 (04/07 ~ 04/09, 대전컨벤션센터) |
| 권호 |
46권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 Ⅰ |
| 제목 |
Development of Cationic Methylcellulose/Zoledronic Acid Nano-complex for Efficient Gene Delivery System and Anticancer Effect |
| 초록 |
Cationic methylcellulose/zoledronic acid nano-complex was developed for efficient gene delivery and anticancer effect. Zoledronic acid (Zol) is typically used for osteoporosis treatments and is known to induce apoptosis in breast cancer cells. Methylcellulose derivative (MCPEI) was synthesized by oxidation of methylcellulose and reductive amination with polyethylenimine. Zol was loaded into MCPEI to form MCPEI/(Zol:pDNA) nano-complex. MCPEI/(Zol:pDNA) nano-complex showed higher transfection efficiency than MCPEI in HeLa cells, due to endosome buffering of imidazole group of Zol. Bafilomycin A1-treated transfection showed significantly decreased efficiency, indicating that transfection is processed by endocytosis. MCPEI/(Zol:pJDK-apoptin) complex showed significant anticancer activity in MDA-MB-231 cells, due to the combinatorial effect of Zol and apoptin. It was concluded that MCPEI/(Zol:pDNA) complex improved the transfection efficiency and showed potential for anticancer treatment. |
| 저자 |
정소희, 이미겸, 김태일
|
| 소속 |
서울대 |
| 키워드 |
Gene Delivery Systems; Zoledronate; Polyethylenimine; Methylcellulose; Endosome Buffer;
|
| E-Mail |
|
