| 학회 |
한국고분자학회 |
| 학술대회 |
2015년 봄 (04/08 ~ 04/10, 대전컨벤션센터) |
| 권호 |
40권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 II |
| 제목 |
NON-VIRAL DIRECT CONVERSION OF PRIMARY FIBROBLASTS INTO NEURONAL CELLS USING BIODEGRADABLE POLYMER NANOPARTICLES |
| 초록 |
Recent studies have shown that defined sets of transcription factors can directly reprogram differentiated cells into another linage without passing through a pluripotent stem cell-like state. Most studies reported so far have used viral vectors to deliver the genes, which causes the safety issues, hence limits the scope of their potential clinical applications. Here, we report a novel method for reprogramming mouse fibroblasts directly into neuronal cells using specific biodegradable polymer nanoparticles. These induced neuronal cells uniformly displayed morphological and molecular features of neuronal cells, and exhibited electrophysiological functions. Our non-viral direct conversion approach may endorse the prospect of autologous cell therapy for neurodegenerative disorder. This work was supported by a grant (HI13C1479) from the Korea Health Technology R&D Project funded by the Ministry of Health and Welfare, Republic of Korea. |
| 저자 |
진윤희1, 조안나2, 박현지2, 이정승2, 조승우2
|
| 소속 |
1연세 대, 2연세대 |
| 키워드 |
Direct conversion; non-viral gene delivery; polymer nanoparticle
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| E-Mail |
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