| 초록 |
Soft, transparent poly (dimethyl siloxane) (PDMS)-based cranial windows in animal models have created many opportunities to investigate brain functions with in vivo imaging modalities. However, the poor wettability by cerebrospinal fluid (CSF) may cause bubble trapping beneath the window during implantation and favorable heterogeneous bubble nucleation at the interface between PDMS and CSF. Herein, to yield biocompatibility-enhanced and trapped bubble-minimized windows, we introduce a soft, CSF-philic PDMS window coated by a poly (vinyl alcohol) (PVA) for long-term in vivo imaging. The PVA-coated PDMS (PVA/PDMS) film exhibits low contact angle (33.7±1.9°) with artificial CSF solution and maintains sustained CSF-philicity to achieve bubble-free window and to induce the formation of thin wetting film that shows anti-biofouling performance for long-term optical clarity. In vivo studies on mice cortex verify that the CSF-philic feature of PVA/PDMS window provides minimal damage to neuronal tissues, and attenuate immune response. These advantages are correlated with the enhancement of hemodynamic changes and local field potentials recorded through the window, respectively. |
