| 초록 |
Haematococcus pluvialishas attracted interest as a bio-platform for producing omega-3 fatty acids (ω-3FA) and astaxanthin that have a great potential as anti-inflammatory drugs.This study aimed to simultaneously improve the CO2fixation and the productivities of ω-3 FA and astaxanthin,via calcium-mediated homeoviscous adaptation and biomineralization. As a resultof 3 mM of Ca2+addition, ω-3 FA content was improved by 31% due to Ca2+-inducedhomeoviscous adaptation. Biomineralization was promoted by the extracellularcarbonic anhydrase, which resulted in 46.3% improvement in CO2fixation. CaCO3from the biomineralization was beneficially re-used in the H. pluvialisculture and triggered 178- and 522-fold increased biomass productivity andastaxanthin content, respectively, thanks to its anisotropic nature. The Ca2+-basedproductivity enhancement strategy was applied to large-scale culture whichresulted improvement in overall bioprocess performance. |
