| 초록 |
When massive or unexpected hemorrhages occur, hemostatic agents are needed to immediately stop bleeding. When skin is injured, the activated platelets release polyphosphate (polyP), which have critical roles in blood clotting cascade, such as triggering the clot formation, promoting the activation of factor V, and enhancing fibrin fibers' thickness. Herein, we conjugated phosphate groups to chitosan polymer backbone to mimic polyP because the longer length of polyP promotes the faster formation of blood clot as well as the hemostatic property of chitosan. The conjugation of phosphates were confirmed by FT-IR and quantified using 31P-NMR analyses. The structure of phosphorylated chitosan was analyzed by 13C-NMR. The phosphorylated chitosan with different amount of phosphates were applied on the bleeding liver, and the hemostatic time for each was measured. As a result, time to hemostasis was reduced as the amount of the conjugated phosphate was increased |
